APPLICATIONS RELATED TO HUMAN HAIR
FIG. 1: INVITRO HAIRFOLLICLE 92 DAYS MATURATED
CMO- TISSUE ENGINEERING (TE) OF 3 D ORGANOIDS FOR THERAPY TESTING AND TRANSPLANTATION INTO THE SCALP
In the role of a HAIROID™️ Contract Manufacturing Organisation (CMO) we produce cell-based materials for medical, cosmetics and industrial applications.
This enables us to obtain developmental data for the upscale and gene-editing of desired traits (pigmentation, scale up technology and production/feasibility studies) in human applications without the usual restrictions of human clinical trails regulations.
With the classical TE approach, cells are seeded onto a prefabricated scaffold, typically in conjunction with the delivery of bioactive factors that ensure maintenance of cellular phenotype and appropriate extracellular matrix formation.
An alternative concept envisions the development of in vitro 3D tissue models, which exhibit functional features of native tissues, for application in an in vitro testing or screening system.
Every human is different, therefore, we all have different susceptibilities to suffer from a particular disease and we all react differently to certain medications.
All HAIROID ™️ approaches are entirely based on human induced pluripotent stem cells (iPSCs). Consequently, they are a priori personalized. While we usually make use of a library of available and well-characterized human iPSCs, we are extending our activities to fully personalized approaches.
IMAGE.2: processing of harvested hairfollicle biopsies
Based on a small FUE sample (an amount of 30 FUExcission obtained hair-follicles) we can generate personalized hair-follicle organoids.
These HAIROIDS will be used for several potential applications-
Alopecia is often diagnosed at a rather late stage of the disease progression. We aim at further developing our hair organoid technology to be able to predict disease susceptibility long before the first symptoms become observable.
FIG. 3: follicle assessment schematics
3D Human hair organoids
The unique feature of our approach is that we use complex 3D human organoids whereas we bioengineer the organ to a level that is not achievable by traditional in vitro cell culture.
Hence, by using the individual's cells we are able to generate on-a-dish a model of the organs. This allows the identification/correction of potential defects and the pre-test of drugs when multiple choices are available.
For these services, we make use of our expertise in the generation of human induced pluripotent stem cells (iPSCs). All our approaches are therefore completely free of any kind of animal testing.
The iPSCs are used to generate human hair/skin organoids for disease modelling, drug testing and transplantation.
Furthermore, our bioengineered hair organoid requires the isolation of Dermal Papillae (DPSC), HB stem-cells, their merging with IPSC and their cryogenic storage.
FIG.4: merger of DP and iPSc into the HAIROID™️