Model description:
RHM is an in vitro reconstructed human oral mucosa consisting of differentiated stratified oral epithelium on a fibroblast populated collagen hydrogel cultured on an inert polycarboanate filter at the air-liquid interface, in a chemically defined medium.
This model is histologically similar to in vivo human oral mucosa.
Specific markers:
Differentiation markers
- Keratins 4, 6, 10, 13, 14, 16, 19
- Loricrin
- Involucrin
Anti-microbial markers
- Elafin Human beta defensins 2 and 3
Lamina propria – epithelial junction markers
Tol-like receptors and signaling
- TLRs 1-9
- MyD88 dependent and independent pathways,
- downstream signaling
Lamina propria markers
Proliferation markers
- Ki67
- PCNA
- MTT assay
- LDH assay
Cytokine, chemokine and growth factor secretion
- e.g.IL-1alpha, IL-6, IL-8, CCL2, CCL5, CCL20, HGF, VEGF
Applications:
Inflammation / irritation / cytotoxicity
Host – microbiome interactions (commensal and pathogenic; single species an biofilm)
Medical devices (leachables, dental implants & abutments)
Wound healing (inflammation, closure, fibrosis)
DNA damage
Advanced methods:
Immunohistochemistry
Western Blot
Fluorescence in situ hybridization (FISH)
Omics
Multiplex arrays
Light, fluorescent, confocal & Electron microscopy (TEM / SEM)
Organ-on-chip
Format:
12 well plate: 1cm diameter
6 well plate: 2.5 cm diameter
