CONTRACT RESEARCH FOR APPLICATIONS RELATED TO THE HUMAN ORAL MUCOSA
RHM is an in vitro reconstructed human oral mucosa consisting of differentiated stratified oral epithelium on a fibroblast populated collagen hydrogel cultured on an inert polycarboanate filter at the air-liquid interface, in a chemically defined medium.
This model is histologically similar to in vivo human oral mucosa.
Lamina propria – epithelial junction markers
Tol-like receptors and signaling
Lamina propria markers
Cytokine, chemokine and growth factor secretion
Host – microbiome interactions (commensal and pathogenic; single species an biofilm)
Medical devices (leachables, dental implants & abutments)
Fluorescence in situ hybridization (FISH)
Light, fluorescent, confocal & Electron microscopy (TEM / SEM)
12 well plate: 1cm diameter
6 well plate: 2.5 cm diameter
Example 1. Oral mucosa wound healing model:
Example 2. Gingiva Irritant potency assay: