CONTRACT RESEARCH FOR APPLICATIONS RELATED TO THE HUMAN ORAL MUCOSA

Model description:

RHM is an in vitro reconstructed human oral mucosa consisting of differentiated stratified oral epithelium on a fibroblast populated collagen hydrogel cultured on an inert polycarboanate filter at the air-liquid interface, in a chemically defined medium.

This model is histologically similar to in vivo human oral mucosa.

Specific markers:

Differentiation markers

  • Keratins 4, 6, 10, 13, 14, 16, 19
  • Loricrin
  • Involucrin

Anti-microbial markers

  • Elafin Human beta defensins 2 and 3

Lamina propria – epithelial junction markers

  • Laminin
  • Collagen IV

Tol-like receptors and signaling

  • TLRs 1-9
  • MyD88 dependent and independent pathways,
  • downstream signaling

Lamina propria markers

  • Fibroblasts: vimentin

Proliferation markers

  • Ki67
  • PCNA
  • MTT assay
  • LDH assay

Cytokine, chemokine and growth factor secretion

  • e.g.IL-1alpha, IL-6, IL-8, CCL2, CCL5, CCL20, HGF, VEGF


Applications:

Inflammation / irritation / cytotoxicity

Host – microbiome interactions (commensal and pathogenic; single species an biofilm)

Medical devices (leachables, dental implants & abutments)

Wound healing (inflammation, closure, fibrosis)

DNA damage

Advanced methods:

Immunohistochemistry

Western Blot

Fluorescence in situ hybridization (FISH)

Omics

Multiplex arrays

Light, fluorescent, confocal & Electron microscopy (TEM / SEM)

Organ-on-chip


Format:

12 well plate: 1cm diameter

6 well plate: 2.5 cm diameter

How we engineer oral mucosa:


Example 1. Oral mucosa wound healing model:


Example 2. Gingiva Irritant potency assay: