CONTRACT RESEARCH FOR APPLICATIONS RELATED TO HUMAN SKIN

Model description:
In modern contract R&D there’s an unmet need for physiologically relevant human skin models. In order to determine whether the substance of interest is safe for human exposure (predict an adverse event), to identify the mode of action, to further identify the target group and for the risk assessment of therapeutics precise engineered tissue models are essential. Human skin and scar models are cultured human skin cells, which serve as an alternative for animal testing and mimic the human reaction in vitro as closely as possible. Geneusbiotech  offers a broad range of state-of-the-art human skin models (for investigating how compounds can interfere with normal skin homeostasis and disease) and skin tissue engineering expertise. The skin models can be adjusted to specific research needs.
We offer various models such as: 

1. Adipose and Dermis is derived MSC, Fibroblasts and Endothelial cells
2. Pigmented skin equivalents, Keratinocytes, Melanocytes and Fibroblasts 
3. Adipose layer equivalent-ASCs populated Hydrogel
4. Immune-competent skin containing Langerhans Cells (CD1a)
5. Scar models (Human Scar, Hypertrophic Scar & Keloid Model)

Read-out options:

Tissue histology (HE, IHC)
Viability (MTT/Trypan blue)
Proliferation, migration and contraction assays
 Apoptosis and Necrosis
Dose finding/Dose Cytotoxicity
Wound-healing factor secretion
Effect of compounds on cytokine, chemokines & growth factor production
Risk assessment of chemicals
 Screening of compounds and chemicals on positive effect on scar quality, reduction, refinement & prevention
Effect of compound/chemical on thickness of dermis, contraction, Collagen 1 secretion, Epidermal cell layers.
Outgrowth of epidermis, IL-6 & CXCL8 secretion

Assays and Techniques e.g:

  • ELISA
  • FACS
  •  Western Blotting
  •  qPCR
  • Specific kits


Applications:
  • Risk assessment of novel drugs and chemicals
  • Physiologically relevant human models understanding scar pathology, for drug targeting and testing novel therapeutics
  • Regenerative medicine aims to restore tissue or organ to original healthy state after disease or damage
  • DNA damage and UV
  • toxicity
  • Irritation
  • Sensitization
  • Wound healing
  • Scar models
  • Topical
  • Injection
  • Systemic (in medium) Time frame exposure e.g: 2, 4, 8, 24, 72 hrs.

Advanced models:
Test substance application method: topical, intradermal, subcutaneous (fat injection in excised skin), systemic (in culture medium)


Fig.2: Immune-competent skin